Do you really identify the authenticity of the ELISA kit? -Huaqiang Electronic Network

Because ELISA (Enzyme Linked Immunoassay) has the characteristics of simple operation, high sensitivity and good specificity, it is deeply loved by researchers and greatly facilitates the work of researchers. However, there must be some small companies that are profitable. , using the fake and inferior elisa kit to harm the majority of scientific research workers. Here, Shanghai Hengyuan will show you how to distinguish the authenticity of the ELISA kit. Falsification 1: Registering a company abroad, domestic production, concealing production origin, it is clearly a domestic reagent, but claims that foreign imports falsely report high prices, deceiving users. Identify the good and bad methods: search for the "factory name", "ELISA" and "fake" keywords on Google. Users of various forums will discuss the quality and effect of the ELISA kit. Counterfeiting 2: Counterfeit imported ELISA kits make the quality of imported ELISAs uneven, mixed with fish, and many of them are processed by small workshops. Such kits can occasionally produce results. If they can't do it, they can buy them. The company does not have perfect after-sales service, and often pushes the three-response to the customer's problems, shirking responsibility, and finally the consumer suffers. Identify the good and bad methods: call the manufacturer directly, ask them the source of the kit antigen and antibody, ask for the kit instructions, and then use the network to confirm this information, the formal ELISA manufacturer, this information is very complete, if the information is not complete The ELISA will have a poor manufacturing level and quality. Fraud III: In order to exaggerate the scope of the indicators covered by the production ELISA, some profiteers use one indicator to impersonate other indicators, resulting in the illusion that various species and indicators can be made. Identify the good and bad methods: I. Use the interference test to identify whether the ELISA test kit detects the antigen of interest. In order to determine the optimal signal and the lowest background in the ELISA experiment, the capture antibody (0.5-4 μg/ml) and the detection antibody (0.25-2 μg/ml) should be titrated against each other in a preliminary experiment. At the same time, serial dilutions of standards should be included in the appropriate range. Operate according to the range generally provided in the reagent manual. Preparation of standards: For each cytokine, read the instructions carefully and pay attention to the details of each batch. Immediately centrifuge the reagent bottle before using the cytokine to recover as much of the cytokine as possible. Lyophilized cytokines were reconstituted according to the details in each batch of instructions. Generally, the linear range of the standard curve of the antigen to be tested can be from 2000 pg/ml to 15 pg/ml by 8 times serial dilution of the standard. Sensitivity can be increased to a certain extent using standard ELISA procedures, amplification kits, reagents of the third type, or changes to the enzyme substrate system. To optimize sensitivity, it is recommended to incubate standards and specimens overnight. If peroxidase is used as the color development system, it is strictly forbidden to add sodium azide to the washing solution and the diluent. The sodium azide can inhibit the activity of the enzyme of the ELISA kit. Our company adheres to the concept of “Credit First, Quality First, Brand First, Honesty Based”. It is recognized by the scientific research institutions of all major universities and designated as Elisa kit standard products with excellent service quality and affordable price. Long-term supplier of reference products. We will serve the majority of scientific research users with the spirit of professional dedication and excellence.