远慕生物整理出- ELISA experiment small coup - Huaqiang Electronic Network

远慕生物整理--ELISA experiment

Newcomers who have just been exposed to ELISA experiments often say that the operation is complicated, the steps are many, and it is not easy to experiment. In fact, when we master the skills and attention to avoid it, it will be much simpler. My company's experts analyzed the operation tips, these experimental skills are well remembered:

1. Incubate in strict accordance with the time indicated in the instructions, the amount of liquid added, and the sequence.

2. Shake well all liquid components before use.

3. Before the experiment, the product should be stored at 2-8 ° C, and equilibrated for 20 minutes at room temperature before use. The concentrated washing liquid taken out from the refrigerator will crystallize, which is a normal phenomenon, and the water bath is heated to completely dissolve the crystals before use.

·Method of evaluation

1. Investigation of quality control substances, which is a common form at home and abroad, using regular quality control materials to each laboratory, each laboratory carries out inspections on the specified date, and reports the results to the organizers (Ministry, Ministry Procuratorate). Through statistical analysis, the organizers send the evaluation results back to the laboratory to understand the quality of work, identify problems, and improve quality. The disadvantage is that the labs do not achieve a true evaluation because they eat small stoves or call each other to modify the results.

2. On-site investigation, without prior notice, temporarily dispatched personnel to the laboratories, stipulated the use of conventional methods to test a group of specimens for evaluation. This method can solve practical problems and conduct on-the-spot guidance. Organizers often cannot perform on a regular basis due to human and material resources.

·Experiment record

1. The original data of all experiments should be archived;

2. All records should be registered in the register;

3. The original registration form should record the source of the reagent and the batch number;

4. The source and measured value of the quality control serum and indicate whether it is under control;

5. Sign the name of the experimenter and the name of the reviewer.

Elisa kit ELISA test kit