Mainly by chromatographic pumps and controllers, injectors, columns,
Detector And the data processing and control of the five major components, the separation principle is a physical process, the mobile phase carries the compound to be analyzed and some other coexisting substances flow through the column, using different substances on the stationary phase retention time is different, so the peak time is different The separation is achieved, and the retention time is used to characterize the peak height or the peak area. When the separated components are sequentially passed through a UV detector, the concentration of each compound can be detected. 
High-performance liquid chromatography has the characteristics of “three highs, one wide, one fastâ€:
1. High pressure: The mobile phase is a liquid. When flowing through the column, the resistance is large. In order to pass the column quickly, high pressure must be applied to the carrier liquid.
2, high efficiency: high separation efficiency. The stationary phase and mobile phase can be selected for optimum separation, many times more efficient than industrial distillation columns and gas chromatography.
3, high sensitivity: UV detector up to 0.01ng, injection volume in the order of μL.
4, a wide range of applications: more than 70% of organic compounds can be analyzed by high performance liquid chromatography, especially the separation of high boiling point, macromolecules, strong polarity, poor thermal stability compounds, showing advantages.
5, the analysis speed is fast, the carrier liquid flow rate is fast: much faster than the classical liquid chromatography method, usually one sample is analyzed in 15 ~ 30 min, some samples can be completed within 5min, generally less than 1h. In addition, HPLC has the advantages that the column can be used repeatedly, the sample is not destroyed, and it is easy to recycle.
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