The effect of background light illumination intensity on retinal electrical and oscillating potentials Wang Ling 1317 Shan 1; 3 changes. Interpretation network. Sigh, the reporting mechanism of the response to the environmental lighting is less responsive to the application of the line design. It is noted that the fiber electrode is at the corner and the yttrium is recorded as 29. 1 rat 4 eyes; and 4 in 2 logarithms, 4 back light intensity Iril.43 10 hours recorded, 3 stimulus flash intensity is 1.4312 plus 2, flash time duration 75 0, flash interval 1 The result is strong with the intensity of the background illumination. The number of wavelets is reduced from 5 to 3. However, the sum of the sub-wave oscillators increases the intensity of the background light to the strongest level. The sum of the amplitudes is reduced. 3. The wave and wave amplitudes are unchanged under 2 weak background illuminations. But in the strong background illumination range, 1 wave and. The amplitude of the wave varies strongly with the intensity of the illumination. In conclusion, the neuroregulatory system present in the inner layer of the rat retina is more sensitive to changes in environmental illumination than the photoreceptor cells. Recording, using appropriate continuous backlighting at 8 o'clock, can improve the stability of the reaction record.
The group frequency of the ascending branch has low-amplitude rhythmic wavelets. So far, our understanding of 8 is far less profound than the understanding of 3 waves and 1 skin, but 08 reverses the inner retina to the photoreceptor cells in the retina. The understanding of sexual regulation mechanism has been widely accepted in 4 circles, 61 1.23 in the study of human eyes, the waveform of 08 changes with the stimulation of the flash interval. The flash interval can adjust the dark adaptation state of the retina when the retina is in the Ming Sight and sneak peek. 31, 1 in the state of 0 people, Liu Jipi 3, the most human 0 reaction, 3 coffee, 1. This and now, the measurement for clinical 08 is based on the study of Boundary 8 such as 6, the study It is proposed to use continuous background illumination with different intensities to observe changes in the mouse eye of different dark adaptation states1. Analysis of the response of the neural retinal mechanism to environmental illumination changes 1 Materials and methods 1.1 Experimental animals were kept under normal lighting conditions under 304 dark-vision lighting conditions.
1.2 The experimental conditions were adopted. One person 2 recorded the values ​​of the electroretinograph with the design of 4, and in parallel, the statistical significance level was 0.05 2. The results showed that the retinal electric power and the oscillating potential were set at a right angle of 2.1 waves with the stimulation light path. The 3 wave changes the illumination path, and the stimulating light and the background illumination light are merged into the same beam by the beam splitter. The amplitude of the 3 waves is about 80, 〃, the wave is 1 light path stimulating light and the back 37 light source are 86 illusion bubbles 6, 15 circles in the experimental animal eye position measured 2 kinds of light source, the intensity of the light is 1.410 1 stimulating light illuminating mode is single flash, flashing time course 75, flashing interval time 5 background light intensity using medium å¿ filter wide attenuation recording by preamplifier team IOAAHeadstage, NL 104AACpreampHfier, Digiti161. Mountain 1 magnified meter. The factory designed the recording data of this ingenious recording system. The 3 wave and 1 wave recording passband is 1 700 out. The digital filter is used to set 4250 out as the vibrating potential recording passband.
1.3 Experimental Procedure All experimental animals were first adapted to 141 tones, under dark red lighting. 135mgmljy, OUVdttKKm atropine dilute sputum recording electrode is 0 fiber electrode, the upper é’ˆ pin type reference electrode, the lower é’ˆ pin type ground electrode 3. After the preparation is completed, the person is placed, the mouse is in the black box only leaving the power supply into the hole Inside. Dark adaptation 5 again, the experimental animals in the dark box were stimulated four times, while the retinal electricity and oscillating potential were recorded, and the reaction of the third and fourth flashes was superimposed as a scotopic reaction to illuminate the background light. The 678th reaction was superimposed on average, and the background light intensity as background light illumination reaction was from 1.4 square ia6cdm2feS2 paying towels 4, respectively, weak weak sub-strong and strong 4 intensity 2 different back light intensity between experimental animals After dark adaptation 30 records the reproducible results with the first vagus, the background light intensity is changed, and all the recorded signals of the above experimental process are stored in the computer for the spin-off 1.4 data analysis and processing; 3 wave amplitude is The high-intensity 1 wave amplitude from the baseline to the 3-wave valley is the height from the 3 wave trough to the 3 wave crest. The amplitude of the kicking potential wavelet is the height of the line connecting the peak of the wave and the point at which the line connecting the wave 1 from the low point intersects. The sum of the amplitudes of the wavelets is called the total oscillation potential rj`AjI80UY. When the background illumination intensity is weak and weak, the tenth; 1; 1 and 1 wave amplitudes are not changed, and the intensity of the illumination is enhanced with the intensity of the lighting. The wave amplitudes all show a downward trend. 1 In the case of sub-strong background illumination, the wave amplitude decreases. 2 The wave amplitude decreases. 41. When the background light intensity increases to the maximum, the wave amplitude decreases by 9 and the chopping amplitude decreases by 7. Unfiltered, filtered ERG backlight illumination intensity logarithmic unit oscillation potential record. The test animals were born 29 = rats, and the middle arrow stimulated the flash to start 301 amplitude. The median value is the average of 5 human brain eyes born to 2,291, the vertical line mean of 9, the confidence interval age, the poor background light illumination intensity logarithm unit summedosrillalorynsKiiiscs, the SOPs training flash starts in 1 wave The time of the trough is. At the peak, the time to the peak is 1 pico. The time during which the excitation flashes to the respective wavelet peaks of the oscillation potential is the sub-peak. Calculate the dark-visible and different-intensity background illumination, respectively, the amplitude and peak time of the 3 and 1 waves, and the 95 confidence interval of the mean and mean of the total amplitude of the oscillatory potential are the same as 2.21; When the cargo is dark and weak, the oscillating potential of the ruler has 5 wavelets. When the sub-strong and strong backlights are illuminated, the oscillating potential has only 3 wavelets 1 in the weak and weak background illumination. The total oscillating potential increases with the increase of background light intensity, and the change of 3 waves and 1 peak at 3 ä¸ is unknown. In the strongest background light illumination, the peak is obviously prolonged, while in the case of 1 peak, the peak is significantly shortened by 4, and the corresponding parameters of the illumination intensity logarithm are taken. The change in the parameter has a statistically significant line mean of 9 smoke confidence interval. 3 Discussion Our results show that the total oscillation potential value is the largest under the sub-strong background illumination, which is consistent with the human 5 such as in the human eye. The results obtained were examined to show that the neurological regulation system of the retina is most active in the dark adaptation state. The background illumination intensity used in this study is flat. It is unclear at what level of dark light adaptation these background light intensities give to the mouse eye. However, it is a bit certain that it is active under the strong background illumination used in this research. Therefore, when recording the oscillation potential of only 0, giving appropriate intensity of continuous backlight illumination will help to record the larger 08 signal. Conducive to the repeatability of the results, reducing the difference between multiple records So far, we know that the dark adaptation of the human eye includes 2 parts.
é‚° is divided into neurological fast, should be systematic. The rapid visual acuity change is now irrelevant to the photochemical response of this photoreceptor. The other part is the slow adaptation system, which is a change of slow visual acuity. In this process, the visual acuity is determined by the content of visual pigment in rod cells! Previous studies have confirmed that the three-wave and wave modes of retinal electricity are closely related to the content of visual pigment in photoreceptor cells. Therefore, the slow-wave threshold component of the ruler is often used for visual acuity, although the human-to-eye slow adaptation system The understanding is relatively mature, and the understanding of the neural crest rapid adaptation system is still poor. Early studies in 17 found that the dark background illumination that does not affect the photoreceptor cells and horizontal cells can cause the sensitivity of the inner layer of the retina to decrease, that is, Depending on the network, he pointed out that the inner retina has a non-photosensitive cellular neurological illumination, the amplitude of 8 waves and 1 wave has not changed, but the oscillation potential is increased to support the above theory, and the progress indicates the neurological adaptation system of the inner layer of the retina. More sensitive than the photochemical reaction system to environmental lighting changes. The essence may be the retinal signal system or the rapid visual control center of the mouse eye when the ambient illumination changes.
The shortening of the 81-wave peak time under strong background illumination in this study has been reported in other scholars' visual electrophysiological articles on Ming adaptation. The lag of 9 and 3 peaks is to suppress the background light to the outer retina, especially the photoreceptor cells. Therefore, it can be imagined that the neuro-regulatory system of the detachment of the visual network emits a timely signal for the change of the ambient illumination, and the mouse eye with a very poor vision system plays a visual protection role in a certain sense.
4 References This article edits Tang Jian
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